of late , the Lily Research Group of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences has revealed the molecular mechanism of anthocyanin exaltation in two - color lily ( ‘ midget Padhye ’ ) . Related inquiry results were publish online in " Genes " .
Lily flowers are not only diverse in color , but also have rich variations in petal coloring types , and have important cosmetic and commercial-grade time value . Anthocyanin is an of import pigment for lily coloration . It is synthesize in the cytol , but it needs to be transport into the vacuole to make the plant color . But so far , there are few reports on the transport mechanism of anthocyanins in lilies . The disclosure of the molecular mechanism of the anthocyanin transport in the two - color lily can provide a theoretical basis for the contrived regularization and molecular improvement of the flower color .
The inquiry used RACE technology , bioinformatics analysis , subcellular localization , complementary experiments and other experimental method acting to consider the role of better half tape transport protein in lily anthocyanin raptus . written report have shown that the protein encode by the LhDTX35 gene in lily is an significant transport membrane protein in the MATE cistron family . Expression profile data psychoanalysis showed that the LhDTX35 cistron was positively correlate with the look of anthocyanins in lily flowers of dissimilar developmental stages , and the gene was extremely expressed in the coloured parts of the lower petals and humbled in the colourless parts of the upper petals at S2 . The complementary experiment of Arabidopsis mutant showed that LhDTX35 factor can doctor the phenotype of Arabidopsis DTX35 mutant pod asepsis and low anthocyanin message in hypocotyls . The experimental results show that the Mate - similar protein encoded by LhDTX35 cistron in lily involve in the transport of lily anthocyanins .
The report take the Institute of Vegetables and bloom of the Chinese Academy of Agricultural Sciences as the first social unit to finish , Xu Hua , Ph.D. student of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences is the first source and research worker Ming Jun and Assistant Researcher Xu Leifeng are the corresponding writer . The research was abide by the National Natural Science Foundation of China , the National Science and Technology Support Project , and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences .
Source : horticulture.cn
